Multiple reasons. Where did you create your project? On a server or your local machine? If it is on your local machine then are you an administrator of that machine?
If it is on a web server then is someone else currently debugging? Only one person can be debugguing at once (at least that's how it was in 1.1).
Also, you can go to the task manager of the server and kill the asp worker process (if it is stuck).
I don't understand what you are trying to do though. Are you trying to debug after you copy the site to a new location, because that's not going to work.
Also, did you check to make sure that the worker process isn't running before you try to debug, and is debugging enabled in the web.config file?
You can't debug on your live server. You need to set up a staging server where you can publish to that lets you debug if you want to debug somewhere besides your local computer.
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