Hi, JBaril
Actually I don't think it is open files or oplocks.
Opportunistic locking does not prevent exclusive access to files - it has the opposite effect of allowing access when it shouldn't, resulting in file or more commonly index corruption when several users are doing concurrent updates.
If I understand correctly, you are unable to lock the file (get exclusive access) with even one user.
I think this is because the Win2k3 server is not set up to support netbios under tcp/ip. This will happen if the server either has a static ip address on the network or the DHCP server does not enable netbios and the feature is not enabled. This is often the case for security reasons.
Are you able to administer the server? If so, look at the advanced button, Wins tab on the tcp/ip protocol properties and see if netbios is enabled. If not, then enable it. Likely (but not sure) you will have to restart the server afterwards (it is Windows, after all).
Jock