>> I don't quite understand if you can have user and computer settings configured in the same gpo
Yes, you can put both users and groups in the same GPO and then configure Conputer Settings that will only affect the Computer Accounts (ie the laptops) and User Settings that will only affect the users. If you have a GPO with only Computer Accounts in there, then it's best to disable the User Settings of the Policy as this will help speed up policy processing.
>> Do I also need the user accounts that will be using the laptop to be in that ou as well?
If you have policies defined unser User Settings then yes - you will need the accounts to be in there also. When a Computer starts up (laptop, desktop, server, etc) you will see "Applying Computer Settings" - this is where it's enumerating through all of the policies that are in all of the GPO's in's contained in (starting at the Domain Level and drilling down through the GPO's) and applying those policies. Likewise, when a user logs in the computer will starting the same thing - this time looking at all the GPO's that the user is in and applying those settings. The one exception to this rule would be if policy inheritance was blocked.
>> If I look under Security Filtering on the policy it has Authenticaed users which I thought would mean all users that logged onto the laptop
Yes - you're right. This is the default setting. But as you pointed out, if the User Accounts aren't under that OU then the GPO will not affect the user and therefore the policy won't get applied.
>> So right now I have a ton of settings applied to that ou but non of them take effect on the laptop
I presume you mean you have a ton of settings configured under User Settings that aren't getting applied? Do user's logon to these laptops with their own accounts or do you have special pooled accounts for these? If there are specially created pooled accounts, is there any reason why you didn't want to include them under the same OU as the laptop computer accounts? Obviously if users use their own accounts to logon to the laptops, I could understand why you woudln't want to apply certain settings normally because I presume the users will be travelling with the laptops so you want to enable things like offline files etc?
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