Yes. Select all with Ctrl-A (or click in the square above the row numbers and left of the column letters) and select Format/Column/Unhide from the menu.
Here is a marco that will do the same as what Pete and Zathra have posted. I recorded (Tools/Macro Record New Marco) the macro using the method Pete posted.
Sub unhide_all()
Cells.Select
Selection.EntireColumn.Hidden = False
End Sub
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