I'm having trouble understanding what you are asking.
Are you creating the PDFs with the virtual Acrobat printer, (if so, from what application?) or trying to print existing PDFs on some sort of real printer?
If the latter, what sort of physical printer are you printing to? Many printers have an option when printing pages 2-up to place a border/dividing line (or not) around each page. This is not an Acrobat question, but a printer question.
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