I have a user (senior manager) that is unable to access our internal financial web application. The web app uses integrated authentication. When attempting to access teh site he receives a http 500 error. If I do a run as on his machine and run internet explorer I can access the site with no issues. If he goes to another machine he can access the site with no issues. I found on the server through the IIS logs there is no username being provided when he attempts to connect. I set a seperate test site on the same server and allowed anonymous access and he can access this fine. After lots of research I have changed the settings in IE for User Authentication and for Enable Integrated Authentication and no success. I have even changed teh MTU size on the network adapter (from a post I found here) with no success. This worked a week ago and no updates have been applied and the user says no changes have been made. I have copied the registry from my user account settings for IE and Internet Settings to his hoping the setting would be included but no luck. Since the user is a senior manager and not very computer savvy he is very against wiping out his profile and starting over. Going to IE6 works because he is prompted to enter credentials but unfortunately several other sites do not work properly in IE6 so this solution does not work either. I am tempted to try IE8 but I am not confident this will help. Any other ideas as to what the issue could be and how I might be able to resolve it?