Nothing! The 7200 will just be a tad faster than the 5400. The drive speeds work independantly. If they are both bare, put the operating system on the faster one.
Micker377 has given you good advice. But age and size could also be a factor. You may want to reconsider if the 7200 spin drive is 10 gigs and 5 yrs old and the 5400 spin drive is 60 gigs and newer.
Still, micker377 has given you good advice as the faster drive is likely to be the newer drive as well, just not 100% for sure in every case, there could be other factors.
Actually it is true that adding a 5400 RPM drive to an IDE system with a drive thats 7200 RPM will in fact slow the system down in varying degree's, but so will many other devices. i.e. CD-ROM, DVD-ROM, Iomega IDE zip drives, LS120's the list is actually quite large.
Replace the 5400 drive drive with another 7200 RPM drive.
Or get an external USB case for under 100 bucks, and use it for backups or an additional drive.
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