How to render the mp4 from mp4 (screen capture) without quality loss

[chuacha]

I’m novice in video making, but I need to make some good quality crisp video tutorials for the internet streaming.
So I’ve stopped on mp4 format, cause it is portable and not heavy.

I have a screen captured video with that tool 1000X750

http://www.screenr.com/

Then I’m adding some effects with After Effect.
I’m exporting that using h.264, because that is what I need in the final version.

The quality after After Effect is great and size is good,
but I need to compose several pieces and add audio, which I’m trying to do in Premier again using h.264.

I need the good quality video, not very heavy, because I need to stream in on the website.. But the problem that Adobe Premier low the quality and I’ve tried so many ways and setting and I still don’t have any idea how the prevent that..

My current setting are:

When I create the project:
Display Format: Timecode
Capture format :HDV
Sequence preset: AVC-Intra 720p 30 fps (I’m not which preset to choose when I capture video from the screen but I have tried almost all of them and just AVC helps me avoid the shaking video )
Pixel aspect Ratio: Square pixels (1.0)

When I export the project:
Format: H.264
TV Standart: NTSC (do I need that if I’m going to use that video just for computer not for TV.. ?)
Frame width: 1000 height:750 (do I need the same frame size as AVC-Intra – 1280X720? )
Frame rate: 29.97 fps
Pixel aspect Ratio: Square pixels (1.0)
Profile: high
Level:5.1
Bitrate Encoding: VBR, 2 Pass
Target Bitrate: 6.06
Maximum Bitrate: 10.68


Please help me to find where I’m wrong.. is that possible with some settings to get no loss quality when I render mp4 to mp4 in Premier?

Thank you guys for any help and sorry if my questions sounds stupid as I’m really novice..