No you don't need to reboot the machine.. if u r changing that with ifconfig it will change the subnet mask on the fly.. if u r changing it with GUI you can restart the network service
as service network restart
fyi, '/etc/rc.d/init.d/network restart' and '/sbin/service network restart' are the same thing. In Red Hat, you can substitute 'service' with '/etc/rc.d/init.d/' to access any of the init scripts.
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